V discussion: all agar plates smeared with specimens resulted in colony growth after a 48 incubation period leading to a supporting conclusion that bacteria is present within a wide spectrum of environmental samples. Culturing of bacteria, see the text dna science: a first course, 2nd edition—lab 2) the single circular chromosome of e coli contains about five million dna base pairs, only 1/600 th the haploid amount of dna in a human cell. Neo/sci® student's guide bacterial transformation lab 8 copymaster: permission granted to make unlimited copies copy use confined to educational purposes within a single school building.
A lab report under the subject of microbiology done as a lab session in josai university, japan during a twinning program on 2014 created by: annisa hayatunn. Biotechnology bacterial transformation lab: the effects of pglo dna on e coli method introduction bacteria transformation is the process of a bacterium absorbing and integrating naked dna located on the surface of their membrane. Unformatted text preview: 1 bacteria lab purpose : observe the amount of bacteria that grows on agar plates after being swabbed with potentially bacteria contaminated qtips, and then compare that growth to the bacteria that appears after infected areas are disinfected with antibacterial wipes. During 702 lab, andrew and kate isolated an ara- mutant, and observed dark blue colonies when they patched this mutant on both lb x-gal kan plates and lb ara x-gal kan plates.
Lab 4 cultivation of bacteria protocols for use of cultivation of bacteria, use of general growth, enriched, selective and differential media, plate pouring, determination of temperature range for growth and. Biology 3b laboratory cultural characteristics of bacteria page 2 of 7 a nutrient agar slants - examine your cultures thoroughly and record your observations in your laboratory worksheet. Use a sterile plastic loop to transfer one or two 3 mm bacterial colonies or an equivalent amount of smaller colonies from the streak plate to the + tube. After inoculating complex tsa and selective sa media with a sample form oral cavity, the bacterial growth occurred in about seventy-two hours at 37 0 c on the tsa plate but not on a sa plate the sample from cat's ear did not appear to grow on any of two media. Unknown lab report unknown number 109 tyler wolfangel april 29, 2014 bio 203-604 introduction the study of microbiology requires not only an academic understanding of the microscopic world but also a practical understanding of lab techniques and procedures used to identify, control, and manipulate microorganisms.
Bacterial transformation lab report: transforming ecoli strains with green fluorescent protein ap biology, mods 19-21 abstract in the transformation lab, we discovered the process of bacterial genetic transformation and how to calculate transformation efficiency. Transformation lab there is going to be a lawn of bacteria seen on the plate this tells us that the bacteria remained viable after undergoing the transformation. Genetic transformation occurs when a host organism takes in foreign dna and expresses the foreign gene in this part of the lab, you will introduce a gene for resistance to the antibiotic ampicillin into a bacterial strain that is killed by ampicillin. The materials needed for this lab were 2 sterile test tubes, 500 μl of ice cold 005m cacl2, e coli bacteria cultures, a sterile inoculating loop, a sterile micropipette, 10 μl of pamp solution, a timer, ice, a water bath, 500 μl of luria broth, a spreading rod, 4 plates: 2 ampicillin+ and 2 ampicillin - , and an incubator.
The transformation lab experiment usinge coli and pfluorogreen maryland loaner lab teacher packet email [email protected] to request a word version of this document. The isolated colonies were incubated overnight at 37°c and inoculated onto new nutrient agar (na) plates, after which pure plates of each type of bacteria were prepared and gram stained followed by the observation of single colonies of bacteria under the microscope. Biol 3702: bacterial unknown skills test page 3 of 4 they will grow on the msa plate, but not the mac plate if you have one gram-positive and one gram-negative bacterium in your mixed culture unknown, then. Unknow bacteria lab report unknown 36 introduction the purpose of this lab was to identify two unknown bacteria from a mixed culture the reason for identification of unknown bacteria was to help students recognize different bacteria through different biochemical tests and characteristics. With regard to the bacterial growth on each plate, there are numerous colonies on both lb/amp and lb/amp/ara plates however, there's a lawn of bacteria on the lb (-) pglo plate.
Purpose: to observe gene expression in real time by performing a genetic transformation procedure on e coli bacteria using a plasmid as a vector if successful, the plasmid will provide the e coli with two new traits. Assignment lab report make sure each of the bold parts below are used as section headings in your lab report the writing should be in a 12 font double-spaced and should not be longer than 4 pages (including the tables. Results both plates were examined carefully after a 48 hour incubation period although the difference between the unwashed and washed cultures was not remarkable, there was a slight reduction in the number of bacterial colonies present on the plates that were inoculated with our clean hands.
For example, as stated above in the study of the effect different antibiotics on bacterial growth, next lab you will be measure the zone of inhibition (killed bacteria or bacteria that did not grow in the presence of the specific antibiotic. The bacteria on the (+) pglo lb/amp plate and the (-) pglo lb plates should be whitish the bacteria on the (+) pglo lb/amp/ara plate should appear whitish when exposed to normal, room lighting, but fluoresce bright green upon exposure to the long-wave uv light. This lab was designed so we can see genetic engineers might use specific genetic markers and genes of interest to find their results those bacteria that did have the gene would appear green or survive on the lb/ amp plate because they took up the ampicillin resistance gene.
Another factor that does not directly influence transformation efficiency but rather the observation of results is how well the bacteria can grow in the agar plates if there is not enough ampicillin in the culture dish, not all of the non-transformed bacteria will die. Microbiology biol 275 2 dr eby bassiri [email protected] appears permanently anchored within a specific site in the bacterial genome and is not. Report your coliform bacteria abundance (average of the two plates) for each medium to the spreadsheet on the central lab computer or blackboard did you grow any coliform bacteria on your plates.